The Mass Spectrometry and Proteomic Core Facility is make cutting edge mass spectrometry technology available to RGCB researchers as well as the wider academic and life science industry community While being a core facility, a major goal of the facility is to become a research environment for multidisciplinary research that utilizes mass spectrometry and other proteomics technologies to understand the disease biology and molecular medicine in the post-genomic era.
RGCB Proteomics Core Facility houses the following instrumentation
Synapt G2-HDMS from Waters
This is a hybrid Quadrupole-Time of Flight (Q-TOF) mass spectrometer
with an extra feature called Ion-Mobility by which compounds can be
further separated and resolved based on their shape. The SYNAPT G2
system features new QuanTof technology that combines an innovative high
field pusher and dual stage reflectron with a novel ion detection system
in an optimised, folded, TOF geometry. Together these features provide a
new dimension of high resolution, exact mass and quantitative
performance uniquely available at acquisition rates that are compatible
with UPLC separations.
NanoAcquity UPLCs from Waters
Proteomic core has one nanoACQUITY UltraPerformance LC® (UPLC® )
System and another nanoACQUITY UPLC® System with 2D Technology.
NanoAcquity UPLC is designed for nano-scale, capillary, and narrow-bore
separations to attain the highest chromatographic resolution,
sensitivity, and reproducibility.
The nanoACQUITY UPLC® System with 2D Technology expands the use of
sub-2-micron particles to achieve high peak capacity separations. This
innovative system effectively uses two-dimensional (2D) UPLC for better
chromatographic resolution of complex proteomic samples by using a dual
reversed-phase (RP) approach.
Hydrogen Deuterium Exchanger (HDX) from Waters
HDX by mass spectrometry opens new windows into the dynamics of
biomolecules by providing information on the relative deuterium uptake
of different conformations of a protein, or locations within a protein.
The nanoACQUITY UPLC™ System with HDX Technology leverages UPLC®
separations and high-resolution MS to answer important questions about
changes in protein conformation including: drug binding to a protein,
protein-protein interactions, and protein folding.
UltrafleXtreme MALDI-TOF/TOF from Bruker Daltonik
This Mass spectrometer combines a MALDI source with a TOF/TOF tandem
mass analyzer to acquire both high resolution peptide mass fingerprint
data and MS/MS spectra of peptide ions. This MALDI-TOF/TOF Combines true
2 kHz speed in TOF mode and 1 kHz in TOF/TOF mode with ultrahigh
performance and extreme flexibility for a broad variety of proteomics
applications. In addition, the smartbeam-II™ laser enables ultra-high
data acquisition speed in both MS and MS/MS at full systems performance
EASY-nLC II from Bruker
The EASY-nLC II is a nano-flow HPLC system tailored to the
requirements proteomics applications. This LC system is used to separate
protein and peptide mixtures for MALDI MS. The system is used in
combination with a MALDI spotter.
Proteineer fcII from Bruker
Proteineer fc II is a MALDI spotter which enables automatic liquid
handling for MALDI preparation of LC separated peptide fractions. It is
designed to deposit fractions eluting from a NanoLC column (Easy-nLC II
) onto a MALDI plate, with the MALDI matrix automatically being added
for offline MS and MS/MS analysis.
MALDI Tissue Imaging Setup from Bruker
It is a device for MALDI imaging sample preparation based on
vibrational vaporization of matrix solution.
Off-Gel Liquid Phase IEF system from Agilent
This Agilent 3100 OFFGEL Fractionator uses a novel isoelectric
focusing technique to achieve excellent Isoelectric point (pI)-based
fractionation. The resulting fractions are in solution, making recovery
for LC/MS analysis much easier.
ProteOn™ XPR36 Surface Plasmon Resonance (SPR) System (Bio-Rad)
The ProteOn™ XPR36 protein interaction array system monitors in a
label-free manner the interaction of biomolecules in real-time using
surface plasmon resonance (SPR) technology. Interactions are monitored
over time by detecting the binding of an analyte flowing in a
microfluidic channel to a ligand immobilized on a sensor chip. This
system generates a 6 x 6 interaction array for the simultaneous analysis
of up to six ligands with up to six analytes.
Experion Microfluidic base-on-Chip Electrophoresis System (Biorad)
This automated platform for nucleic acid and protein analysis
incorporates LabChip technology into an integrated system that performs
multiple electrophoresis steps in one. It automatically performs all the
steps of gel-based electrophoresis (sample separation, staining,
destaining, imaging, band detection, and even some data analysis) to
deliver reproducible separation and quantitation results in just 30
minutes. Software analysis tools are offered for flexible data analysis.
The Proteomic Core will create a sample preparation laboratory to
support scientists in processing their samples before the mass
spectrometry analysis. The facility will also maintain an independent
workstation dedicated for Mascot server, and 2 separate data stations
dedicated for proteomics data processing.
Proteomics Core Facility @ Campus 3
The Proteomics Core Facility (PCF) offers LC-MS/MS analysis, including a broad range of services, from mass confirmations to large-scale quantitative proteomic screens, along with all the associated bioinformatics, for clients on and off camps.
Mass spectrometry instrumentation available:
AB SCIEX 3200 QTRAP® LC/MS/MS System
The hybrid quadrupole/linear ion trap technology of the 3200 QTRAP®
System provides the ability to identify and quantitate components from
complex samples in a single run. Triple quadrupole specificity and
quantitation combine with ion trap full-scan MS/MS sensitivity for
simultaneous qualitative and quantitative results.
Rajiv Gandhi Centre for Biotechnology (RGCB), Thycaud Post,
Poojappura, Thiruvananthapuram - 695 014, Kerala, India +91-471-2529400
| 2347975 | 2348753 +91-471-2348096