This core facility provides flow cytometric services and support for the use of flow cytometric techniques. We are equipped with BD FACS Aria II 14 color system and BD FACS Aria III 16 color system. We provide cytometric services required for a vast array of applications.
| SL No: | Service | Service Charges | ||
|---|---|---|---|---|
| RGCB Users | Clients from Academic Institutions | Clients from the Industry or Overseas | ||
| I | Cell cycle analysis: Different cell lines after exposure to test compounds will be stained with propidium iodide and distribution of cells in various phases of cell cycle will be analysed through FACS. |
1000 per sample | 2000 per sample | 4500 or ($75) per sample |
| One concentration of compound in triplicate/time point/cell | ||||
| II | Cell sorting facility: We also provide sterile and non-sterile sorting of various cell types at sorting rates of 20000 cells/second. |
1000 per hour | 3000 per hour | 6000 or ($100) per hour |
| III | Screening for proteasome inhibitors: This is a high-throughput image based screening strategy developed to identify specific proteasome inhibitors using stable cells expressing proteasome sensor vector. We have developed two cancer cell lines HCT116 and U20S cells which stably express the proteasome sensor so that lead molecules can be screened either by automated microscopy or by flow cytometry. |
2000 | 3000 | 6000 or ($100) |
| Four concentrations of compound in triplicate/time point/cell/sample | ||||
