This includes facilities for wide field microscopy, Time lapse microscopy, confocal microscopy and spectral confocal microscope with resonance scanner.
SL No: | Service | Service Charges | ||
---|---|---|---|---|
RGCB Users | Clients from Academic Institutions | Clients from the Industry or Overseas | ||
I |
Apoptosis (Live cell assay) : Both 2D and 3D models : Our
live cell assay measures the ability of the drug to induce caspase
activation in cancer cells using stable cells expressing FRET probe
for activated caspases. We have cells engineered with a FRET probe in
the nucleus so that automated segmentation and analysis is quite easy
to perform using high-throughput imagers. The following validated
cell lines are available with stable expression of FRET probe NLS.
(More will be included in the list soon). |
2000 | 3000 | 6000 or ($100) |
Four concentrations of compound in triplicate/time point/cell/sample | ||||
II |
Assays for Autophagy and screening of m-TOR pathway inhibitors:
Induction of autophagy and inhibitors of m-TOR pathway is also
emerging as an important target in cancer. We have generated stable
cells expressing LC3 EGFP and its translocation to autophagic
vacuoles is used as the readout by imaging approaches and is adapted
for high-throughput image based drug screening. |
2000 | 3000 | 6000 or ($100) |
Four concentrations of compound in triplicate/time point/cell/sample | ||||
III |
Mitophagy inducers or inhibitor screening: The strategy
employs high-throughput image based approach using stable cancer cell
lines expressing sensors of Mitophagy. |
2000 | 3000 | 6000 or ($100) |
Four concentrations of compound in triplicate/time point/cell/sample | ||||
IV |
Angiogenesis |
|||
Method1: We have immortalized human micro- vascular endothelial cells that form quantifiable angiogenic tubules on matrigel. Hence inhibition of tubule formation can be used as the read out for activity. Since the cell line is immortalized, available for screening of large number of compounds using an image based system is feasible. |
2000 | 3000 | 6000 or ($100) | |
Four concentrations of compound in triplicate/time point/cell/sample | ||||
Method2: Rat aortic ring assay: Dorsal aortic rings will be prepared from rat aorta will be placed in a collagen pre-coated 96-well plates and treated with compounds of interest. Rings will be analysed through phase-contrast microscopy to study microvessel out growth. |
3000 | 4000 | 9000 or ($150) | |
Four concentrations of compound in triplicate/time point/cell/sample | ||||
V |
Assay for antioxidant potential using mammalian cells:
Nuclear factor erythroid 2-related factor 2 (Nrf2) is the master
transcription factor of the antioxidant response element pathway,
coordinating the induction of detoxifying and antioxidant enzymes.
Nrf2 is normally sequestered in the cytoplasm by Kelch-like
ECH-associating protein 1 (Keap1) and upon activation tans ocate to
nucleus. We have developed NRF2 EGFP stable cells so that nuclear
translocation can be used as a platform for initial screening. An
approach for identification of strong antioxidants that break the
interaction is also available and utilizes FRET approach. |
2000 | 3000 | 6000 or ($100) |
Four concentrations of compound in triplicate/time point/cell/sample | ||||
VI |
Endoplasmic reticulum calcium releasing compounds: We also
developed high throughput calcium imaging assay based on a FRET-based
calcium indicator at single cell resolution for compound screening. |
2000 | 3000 | 6000 or ($100) |
Four concentrations of compound in triplicate/time point/cell/sample | ||||
VII |
Tumor stem cell assays:Tumor stem cells contribute both for
tumor initiation and tumor drug resistance forming an ideal target in
cancer. However, they are extremely rare in cancer cells and so
difficult to be used for drug screening. We have designed several
assays to study the effect of compounds on tumor stem cells. From
established cell cultures and surgically dissected tumor samples, we
have optimized conditions to isolate stem cell like cells by FACS
which can be used for identifying compounds that specifically target
tumor stem cells using 2D and 3D culture system The spheroid
formation assay can also be used to study cancer stem cells. Here
cells will be plated in low attachment plates and then treated with
compounds of interest. Cells will be observed under phase-contrast
microscope to analyse spheroid formation. Stem cells will form
spheroids under these conditions and effect of compounds on spheroid
formation will be analysed. |
3000 | 4000 | 6000 or ($100) |
Four concentrations of compound in triplicate/time point/cell/sample |