Profile Banner

DBT-SAHAJ Lifetime imaging Facility

FRET /Lifetime Resources available from the facility

BFP- CFP FRET Pairs and Cells
  1. HeLa stably expressing Tag BFP2- CFP NLS inflammatory sensor cells
  2. SiHa stably expressing Tag BFP2- CFP NLS Inflammatory sensor cells
  3. MCF-7 stably expressing Tag BFP2- CFP NLS Inflammatory sensor cells
ECFP- EYFP FRET based sensors and sensor cell lines
  1. HeLa: Cell death sensor for caspase 3
  2. SiHa: Cell death sensor for Caspase 3
  3. U251: Cell death sensor for Caspase 3
  4. MCF7: Cell death sensor for Caspase 3
  5. DLD 1: Cell death sensor for Caspase 3
  6. SiHa cell line pH Lemon: A pH sensor cells
  7. SiHa pH Lemon Mito: A mitophagy sensor cell
  8. SiHa pH Lemon ER: An ER phagy sensor
  9. Ovvar 8 pH Lemon ER: An ER phagy sensor
  10. MCF 7 pH Lemon ER: An ER phagy sensor
  11. BAX cerulean - PARKIN EYFP stable cells
Neongreen - Td Tomato FRET Pairs
  1. HeLa neon Green - Td Tomato NLS caspase 1 sensor cells
  2. SiHa neon Green Td Tomato NLS Caspase 1 sensor cells
  3. Stable cells expressing neon Green NRF2 /Keap 1 mCherry

For imaging service using these resources, please contact Dr. T.R.Santhosh Kumar at trsanthosh@rgcb.res.in