The primary function of Animal Research Facility (ARF) is to assist investigators in their obligation to plan and conduct animal experiments with the highest scientific, humane and ethical principles. This is achieved by development and maintenance of a comprehensive, high quality animal care program, complies with all guidelines and laws concerning with experiments on animals. All studies involving laboratory animals will require prior approval of Institutional Animal Ethics Committee (IAEC) and experiments will strictly follow the rules and regulations laid down by CPCSEA. The animal research facility is equipped with facilities for housing transgenic and immune deficient animals and an in vivo imaging system (IVIS).
|SL No:||Service||Service Charges|
|RGCB Users||Clients from Academic Institutions||Clients from the Industry or Overseas|
Angiogenesis: Tumor specific capillary formation assay: This is an in vivo assay where tumor cells will be injected intradermally to mouse followed by the administration of compound of interest. At day 10 the skin on ventral side will be collected and tumor directed capillaries will be counted.
|3000 per sample||5000 per sample||11,000 or ($180)|
|The study will have two groups; control (treated with vehicle; n=5) and treated (one concentration of compound, n=5).|
Tumor stem cells (in vivo assays):In vivo tumorigenic potential: Sorted stem cells will be injected to the nude mouse to evaluate its tumorigenic potential.
|40,000||60,000||100,000 or ($1800)|
|The study will have minimum of 3 nude mice/cell type.|
Pre-clinical tumor models: We have developed several in vivo tumor model systems for pre-clinical validation of compounds. Available tumor models are listed below and we are in the process updating the list
|Studies involving nude mice: n=5 mice/group/tumor model (minimum two groups)||50,000||70,000||80,000 or ($1400)|
|Studies involving nude mice: n=5 mice/group/tumor model (minimum two groups): : with IVIS imaging||50,000||70,000||80,000 or ($1400)|
|Studies involving regular mice (BALB/c or C57Bl/6) for syngeneic tumor models: n=5 mice/group/tumor model (minimum two groups) : with IVIS imaging||4000||8000||10000 or ($175)|
|Studies involving regular mice (BALB/c or C57Bl/6 or swiss albino) for chemical carcinogenesis models: n=5 mice/group/tumor model (minimum two groups)||5000||8000||10000 or ($175)|
|Studies involving rats (Wistar or Sprague-Dowly) for chemical carcinogenesis models: n=5 mice/group/tumor model (minimum two groups)||8000||12,000||15,000 or ($250)|
Toxicity profiling (ADME/PK studies): This facility is equipped with provision to study toxicity profile of compounds in mice or rat models. Based on client requirement we can conduct acute/sub-acute or chronic toxicity studies.
|will be calculated based on the nature of the study, groups involved, choice of the animals (mice or rat) and parameters accessed.|
Autoimmune and inflammatory disease models:
|Studies involving regular mice (BALB/c or C57Bl/6 or swiss albino) for arthritis and sepsis models: n=5 mice/group (minimum two groups) :||5000||8000||10000 or ($175)|
|Studies involving rats for arthritis models: n=5 rats/group (minimum two groups) :||8000||10,000||15,000 or ($250)|