The Core will provide to both internal and external users at a service cost, high-sensitivity and high-resolution mass spectrometry analysis for protein and peptide characterization as its primary technology. The services offered will be:
Basic mass analysis of peptides and proteins: Using
MALDI-TOF/TOF and/or Waters LC/MS/MS (Q-TOF)
Protein identification with MSMS sequencing: Analysis could
be done by using MALDI-TOF/TOF and/or Waters LC/MS/MS (Q-TOF). Protein
identifications are made using state-of-the-art database search engines
running on dedicated servers, with the capability of searching standard
or custom protein databases.
Post translational modification analysis: The ETD capability
of Waters Synapt G2 HDMS is an extremely powerful tool for identifying
and characterising post-translational modifications, as well as the
precise site within a protein where the modification occurs.
Protein relative quantitation: Protein quantitation can be
accomplished using a 'gel free, label-free' technology which provides
both relative quantitation (test vs. control). Alternatively,
isotope-coded (labeled) samples can be analyzed to provide relative
quantitation information. The analysis will be done on Waters LC/MS/MS
Protein-Protein Interaction Analysis: Protein interaction
analysis will be performed by using BioRad ProteonXPR system and Waters
NanoUPLC coupled HDX manager.
Rajiv Gandhi Centre for Biotechnology (RGCB), Thycaud Post,
Poojappura, Thiruvananthapuram - 695 014, Kerala, India +91-471-2529400
| 2347975 | 2348753 +91-471-2348096